Coding

Part:BBa_K5111005:Design

Designed by: Angus Lusty   Group: iGEM24_City-of-London-UK   (2024-09-25)


CDSs for NosDFY and NosL


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2171
    Illegal BglII site found at 2959
    Illegal BamHI site found at 1784
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 141
    Illegal NgoMIV site found at 336
    Illegal NgoMIV site found at 940
    Illegal NgoMIV site found at 2034
    Illegal NgoMIV site found at 2753
    Illegal NgoMIV site found at 2971
    Illegal NgoMIV site found at 3114
    Illegal AgeI site found at 3194
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Coding sequences were codon-optimised for E. coli and illegal restriction sites were removed.

Please note, the CDS for NosD starts with the GTG codon.



Source

Sequences in the part are derived from P. Stutzeri Zobell


References